Page 676 - WSAVA2018
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 25-28 September, 2018 | Singapore
WSVA8-0097
FELINE MEDICINE
SUITABILITY OF COMMERCIAL FELV NUCLEIC ACID DETECTION KITS FOR SMALL LAB ENVIRONMENTS
S. Maurice1, L. Small2, L. Hazanov3
1Biogal Galed Labs Acs, Research and Development, Galed, Israel 2Biogal. Galed Labs, Marketing, Galed, Israel
3Biogal. Galed Labs, Research and Developement, Galed, Israel
INTRODUCTION
Feline leukemia retrovirus (FeLV) causes a chronic infectious disease manifested by profound anemia, malignancies, and immunosuppression. If present
in multi-cat environments, FeLV has an impact on survival and quality-of-life, therefore accurate and early detection is imperative to prevent the virus from spreading to other cats.
OBJECTIVES
Comparison of two commercially available molecular kits for the detection of FeLV RNA and DNA using several well-known nucleic acid extraction kits.
WSVA8-0182
FELINE MEDICINE
CAUSES OF DEATH OF DOMESTIC CATS IN A VETERINARY HOSPITAL OF MINAS GERAIS STATE, BRAZIL: A RETROSPECTIVE STUDY
A.A. Medeiros-Ronchi1, S.C. Rodrigues1, T.A. Moreira1, F.G.O. Nascimento1, W.T. Blanca1, R.R. Souza1
1Federal University of Uberlandia, Animal Pathology, Uberlandia, Brazil
INTRODUCTION
Cats play an important role as pets in the present society. The knowledge about their main diseases and causes
of death is fundamental to prevent various pathologies. However, when dealing with domestic cats there are very few researches in Veterinary Medicine.
OBJECTIVES
We identified the causes of death in cats through a retrospective study in the Animal Pathology Laboratory in the Federal University of Uberlândia (UFU) (Brazil), in a period of 36 years, in which 350 cat necropsies were conducted.
METHODS
Necropsy medical records of cats were selected
from the archives of Animal Pathology Laboratory (UFU) (Brazil, MinasGerais State). Epidemiological
data as gender, age, and breed were collected. The necroscopic reports were evaluated and the cause
of death recorded. The diagnostic method used was anatomopathological examination, considering all the complementary postmortem examinations performed for definitive diagnosis (histopathological, toxicological, and immunohistochemical). Only definitive diagnosis was considered, and when elucidating the cause of death was not possible, the diagnosis was considered inconclusive.
RESULTS
The most frequent causes of death were digestive system diseases (13.71%), and among them, feline hepatic lipidosis was the most frequent etiology (18.75%). The second most affected system was the respiratory (10.86%) and acute pneumonia, pulmonary edema, and diaphragmatic hernia were the main diseases in this system. Other decease causes were rabies, squamous cell carcinoma, fibrous osteodystrophy, fractures and traumatism, hypovolemic shock, and feline lower urinary tract disease.
CONCLUSIONS
In conclusion, the digestive system was the system most frequently related to the death of cats, and feline hepatic lipidosis was the most frequent disease.
    METHODS
Molecular kits, Biogal PCRun® and PrimerDesign were tested and compared to each other to define the sensitivity, specificity, and suitability for use in small laboratory settings. DNA and/or RNA (N=121) were purified from three different retrospective sample archives (buffy coat and plasma) collected in Switzerland (Group 1), Portugal (Group 2) and USA (Group 3) employing extraction kits from 2 different companies (Qiagen and Zymo). Final results were compared to the Cq values or SNAP FeLV/FIV generated by the laboratory source.
RESULTS
Group 1: PCRun results correlated better with TaqMan (sensitivity 100% / specificity 84%) than with SNAP.
Group 2 Extraction methods affected final results. Zymo preps generated DNA with lower inhibition of the PCRun® reactions (Sensitivity: Zymo 92-97%; Qiagen 74%). Zymo produced poorer quality RNA extracts compared to Qiagen (Sensitivity 89.5-91.5%).
Group 3: Results obtained with PCRun® were 100% complementary to the TaqMan and matched closely to the viremic stages of the subjects.
CONCLUSIONS
PCRun® is a point-of-care test, while PrimerDesign is a Taqman RealTime PCR The hands-on application and accuracy of PCRun® makes it highly suitable
for screening of FeLV in laboratories with minimal equipment.
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43RD WORLD SMALL ANIMAL VETERINARY ASSOCIATION CONGRESS AND 9TH FASAVA CONGRESS

























































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