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 25-28 September, 2018 | Singapore
WSVA8-0061
INFECTIOUS AND EMERGING DISEASES
GENETIC CHARACTERIZATION OF CANINE RESPIRATORY CORONAVIRUSES IN THAILAND, DURING 2016-2017
K. Charoenkul1, T. Janetanakit1, N. Bunpapong2,
S. Boonyapisitsopa1, R. Tangwangvivat1, A. Amonsin1
1Chulalongkorn University, Center of Excellence for Emerging and Re-emerging Infectious Diseases in Animals, Bangkok, Thailand
2Chulalongkorn University, Veterinary Diagnostic Laboratory, Bangkok, Thailand
INTRODUCTION
Emerging canine respiratory coronavirus (CRCoV) causes mild to severe respiratory disease in dogs worldwide The genetic analysis reveals that CRCoV is similar to human (HCoV) and bovine coronavirus (BoV) and posed ability to interspecies transmission In Thailand, the genetic information of CRCoV is limited.
OBJECTIVES
This study aimed to characterize CRCoVs in dogs in Thailand during January 2016 - December 2017
METHODS
From January 2016 to December 2017, 375 nasal swab samples were collected from dogs with respiratory signs. The samples were examined for CRCoV by using RT-PCR assay. Selected RNA samples were subjected to spike (S) gene sequencing based on age, breed, severity of clinical signs, collection date and location.
RESULTS
In this study, occurrence of CRCoV was 8.8 % (33/375) Five Thai CRCoVs were selected for S gene sequencing Our results show that nucleotide identities of Thai CRCoVs (THA1-CRCoV) and CRCoV-BJ232, HCoV- OC43 and BoV-Quebec were 99.8%, 97.1% and 97%, respectively (Table 1). Phylogenetic analysis showed
that all Thai CRCoVs belong to canine Asian lineage of betacoronavirus (subgroup A coronavirus) which were grouped in the same cluster with human coronaviruses and bovine coronaviruses (Figure 1).
CONCLUSIONS
CRCoV is circulating in dogs in Thailand and Thai CRCoVs were closely related to CRCoV Asian strains, HCoVs and BCoVs. Furthermore, whole genome sequencing is in process for better understanding of the viral diversity and evolution.
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43RD WORLD SMALL ANIMAL VETERINARY ASSOCIATION CONGRESS AND 9TH FASAVA CONGRESS














































































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