Page 696 - WSAVA2018
P. 696

 25-28 September, 2018 | Singapore
WSVA8-0069
INTERNAL MEDICINE (OTHER)
TIME-COURSE EVALUATION OF 2,3-DIPHOSPHOGLYCERATE LEVELS IN CANINE PACKED RED BLOOD CELL STORED IN TWO DIFFERENT ADDITIVE SOLUTIONS
S. Pairor1,2, J. Thanakansakul2, P. Chanloinapha2, S. Sommart2, P. Kongjun2, C. Khumphiranon2,
C. Lekcharoensuk1
1Kasetsart University, Department of Companion Animal clinical Sciences- Faculty of Veterinary Medicine, Bangkok, Thailand
2Kasetsart University, Kasetsart Pet Blood Bank- Kasetsart University Veterinary Teaching Hospital, Bangkok, Thailand
INTRODUCTION
The 2,3-diphosphoglycerate (2,3-DPG) is an allosteric modulator of hemoglobin which facilitates the release of oxygen to tissues. Depletion of 2,3 DPG during RBCs storage is associated with a decreased release of oxygen from the transfused RBCs. Previous studies demonstrated benefits of using additive solutions in order to improve maintenance of 2,3-DPG in human RBCs during storage.
OBJECTIVES
To evaluate the changes of 2,3-DPG levels in canine RBCs stored in two different additive solutions including SAGM and AS-3.
METHODS
Six healthy dogs were included in this study. A unit of whole blood-derived pRBC was prepared from each dog and divided equally into three portions. The first portion was resuspended with SAGM (SAGM group), the second portion was resuspended with AS-3 (AS-3 group), and the third portion was not resuspended with additive solution (control group). The characteristics of SAGM and AS-3 are shown in Table 1.
All pRBC groups were stored in 4oC and samples were obtained at day 1, 14, 21, 28, 35 and 42 of storage. The 2,3-DPG levels from each sample were determined by enzymatic assay kit.
RESULTS
The 2,3-DPG levels were significantly decreased
(p < .001) overtime in all groups (Table 2). The overall 2,3-DPG decline rates were significantly slower in SAGM and AS-3 groups compared to control (p < .05). There were no significant differences in the 2,3-DPG levels and decline rates between SAGM and AS-3 groups (Figure 1).
CONCLUSIONS
These results indicate that SAGM and AS-3 additive solutions provide better maintenance of 2,3-DPG levels in canine pRBCs during storage.
       694
43RD WORLD SMALL ANIMAL VETERINARY ASSOCIATION CONGRESS AND 9TH FASAVA CONGRESS













































































   694   695   696   697   698