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WSVA8-0083
INTERNAL MEDICINE (OTHER)
BABESIOSIS INDUCED EVAN’S SYNDROME (IMHA AND ITP) IN TWO COCKER SPANIEL PUPPIES.
A.K. Sadasiva Muthu1, B.G. Rengaswamy1, S. Baranidharan2, A.H. Mohamed3
1The Ark Veterinary Clinic, General Practice, Chennai, India 2The Ark Veterinary Clinic, General Medicine, Chennai, India 3The Ark Veterinary Clinic, Internal Medicine, Chennai, India
INTRODUCTION
Immune-mediated haemolytic anaemia (IMHA) are
the most common cause of haemolytic anaemia and immune mediated thrombocytopenia (ITP) is the most common cause of severe thrombocytopenia in dogs. In combinations they are known as Evans syndrome, an autoimmune disease in which dogs antibodies attack their own red blood cells and platelets and often fatal.
OBJECTIVES
To successfully management. Evan’s syndrome secondary to canine babesiosis.
METHODS
Two male Cocker spaniel puppies of age 6 months
of the same litter were presented in our practice with profound anemia and thrombocytopenia with recent clinical signs of hematuria, fever, lymphadenopathy, petechiae, echymosis and purpura. Routine clinical findings included blanched mucous membranes, pounding pulses and lethargy. Blood routine revealed marked leukocytosis(W.B.C > 23,000 cells/cmm, severe anemia (Hb<2.9 g/dl) and thrombocytopenia (platelets <14,000 cells/cmm. Coombs in both the dogs showed negative results, the saline agglutination tests showed autoagglutination and the presence of spherocytosis was evident. Flow cytometrical analysis confirmed IMHA. PCR and blood smears confirmed Babesia gibsoni. Treatment with Diaminazene, doxycycline and prednisone were initiated.
RESULTS
One of the pups showed recurrence of anemic hypoxia and thrombocytopenia within 48 hours and was transfused again with DEA1.1 negative whole blood. Both the pups responded well to combination of Doxycycline @ 10 mg per kg sid, prednisone@ 1m mg per kg tapered to 0.25 mg per kg for 2 and Clindaymicin 15 mg per kg bid for 2 weeks.
CONCLUSIONS
This abstract reports the uncommon Evan’s syndrome secondary to canine babesiosis and its successful management with blood transfusion and combination therapy.
Your Singapore, the Tropical Garden City
WSVA8-0114
INTERNAL MEDICINE (OTHER)
ANTIMICROBIAL EFFICIENT OF CHLORHEXIDINE/ CHITOSAN ANTISEPTIC GEL IN VITRO
E. Spucyte1, G. Zamokas1, A. Kavaliauskas1, A. Grigonis1, D. Svetikiene1, L. Babickaite1
1Lithuanian University of Health Sciences, Veterinary Academy, Kaunas, Lithuania
INTRODUCTION
Increasing bacterial resistance to antibiotics is a worldwide problem, which is why external-use antiseptics are gaining attention. Chlorhexidine is one such antiseptic, it reacts with the microbial cell surface. Chitosan increases the permeability of the membranes.
OBJECTIVES
Aim of this study was to assess antimicrobial efficacy of different concentration of chlorhexidine/chitosan gel in vitro.
METHODS
Chlorhexidine/chitosan gel was carried in vitro using
the diffusion agar method. Reference and wild strains
of Gram-positive microorganisms: Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 29212); as well as reference and wild strains of Gram- negative microorganisms: Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) were sown to Mueller-Hinton agar ((CM 0337) Oxoid LTD, Basingstoke, Hampshire, England).Three different concentration chlorhexidine/chitosan gel (0.25 %; 0.5 % and 1.0 %) were tested against each strain of organism.
RESULTS
The gel was active against all bacteria, but effectiveness was dependent on specie and strain. The greatest activity was noticed against the reference strain of S. aureus (diameter of sterile zone from 26,90±0,61 mm
to 29,65±0,30 mm). (c.f. Figures 1 – 2). No significant difference between 0.5 % and 1.0 % concentrations.
CONCLUSIONS
Different concentrations of chlorhexidine/chitosan gel appeared to be effective against all bacteria strains. Whilst the increase in concentration from 0,25% to 1% increased the sterile zone in almost all cases.
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