Page 703 - WSAVA2018
P. 703

WSVA8-0174
ONCOLOGY - ONCOSURGERY
EVALUATION OF LIQUID BIOPSIES TO DETECT CIRCULATING TUMOR DNA IN DOGS WITH CUTANEOUS MAST CELL TUMORS
I.M. Langohr1, K.E. Ballard2, K.O. Strother3, A.F. Roy1, B.B. Boudreaux4
1Louisiana State University School of Veterinary Medicine, Pathobiological Sciences, Baton Rouge, USA
2Louisiana State University, School of Veterinary Medicine, Baton Rouge, USA
3Louisiana State University, Louisiana Animal Disease Diagnostic Laboratory, Baton Rouge, USA
4Louisiana State University School of Veterinary Medicine, Veterinary Clinical Sciences, Baton Rouge, USA
INTRODUCTION
Mast cell tumor (MCT) is the most common skin tumor affecting dogs, therefore improved diagnosis and prognosis of MCTs is crucial. Approximately 20% of MCTs are positive for a c-kit mutation. Such tumors exhibit more aggressive behavior but on the other hand can
METHODS
Cutaneous MCTs were removed and blood and urine were collected from eleven dogs; liquid biopsies were also collected from five healthy, control dogs. Qiagen QIAamp® kits were used to extract DNA from tissue samples and liquid biopsies, and gel-based PCR was completed for detection of c-kit mutation.
RESULTS
c-kit gene was detected in all biopsy samples. It was also detected in the plasma of all dogs, including the control group, and in urine of 80% of the dogs with MCTs. Two of the eleven dogs with MCTs were positive for the mutation in biopsy samples, but c-kit mutation in the corresponding liquid biopsies was not detected.
CONCLUSIONS
The detection of circulating c-kit gene in plasma was successful; however, with the methods used in this study, it was not beneficial for diagnosis. Detection of the mutation in liquid biopsies was not accomplished.
Your Singapore, the Tropical Garden City
WSVA8-0107
ONCOLOGY - ONCOSURGERY
DOG MASTOCYTOMA AND MAMMARY TUMORS FATTY ACID PROFILE: POSSIBLE IMPLICATIONS FOR TUMOR GRADING AND PROGNOSIS
T. Masek1, K. Severin2, I.C. Sostaric-Zuckermann3, M. Mauric4, A. Musulin5, D. Vnuk4, S. Vince6, I. Ljolje7, Z. Ciric8, P. Dzaja2, K. Starcevic4
1Faculty of Veterinary Medicine University of Zagreb, Department of Animal Nutrition and Dietetics, Zagreb, Croatia
2Faculty of Veterinary Medicine University of Zagreb, Department of Forensic and Judicial Veterinary Medicine, Zagreb, Croatia
3Faculty of Veterinary Medicine University of Zagreb, Department of Veterinary Pathology, Zagreb, Croatia
4Faculty of Veterinary Medicine University of Zagreb, Department of Animal Husbandry, Zagreb, Croatia
5Faculty of Veterinary Medicine University of Zagreb, Surgery- Orthopaedics and Ophtalmology Clinic, Zagreb, Croatia
6Faculty of Veterinary Medicine University of Zagreb, Reproduction and Obstetrics Clinic, Zagreb, Croatia
7Veterinary Clinic Buba, Veterinary Clinic Buba, Zagreb, Croatia 8Veterinry clinic VetPoint, Veterinry clinic VetPoint, Zagreb, Croatia
INTRODUCTION
Lipid metabolism has been accepted as a major metabolic pathway that is involved in many aspects of cancer cell pathogenesis. Attempts to understand the role of different lipids in cancer physiology depend on the ability to accurately monitor alterations in lipid composition at cell level due to the diversity of lipid classes.
OBJECTIVES
Mastocytoma and mammary gland tumor are common tumors in pet veterinary medicine. We investigated the differences in fatty acid profile of tumor and normal tissue sample in attempt to get better insight into the lipid metabolism of tumor cells as well as to improve grading of studied tumors.
METHODS
Paired samples of tumor and adjacent non-tumor tissue were isolated from 21 mastocytoma and 37 mammary tumors. After the pathohistological examination of tumor and non-tumor samples, fatty acid profile was determined by GC-MS.
RESULTS
All tumors showed significant difference compared to the normal tissue. The most significant differences were the increased content in C20:4n6 in both tumors, the increased content in 18:0, 18:1n9, 22:4n6 and 22:5n3
for mastocytoma and the increased content of 18:1n7, 20:3n6, C22:4n6 and C22:5n6 for the mammary tumors.
    be specifically targeted by tyrosine kinase inhibitors. Detection of the mutation currently requires testing on tissue samples. Detection of c-kit gene and mutation in liquid biopsies would provide a less invasive technique for diagnostic, therapeutic and prognostic purposes.
OBJECTIVES
Evaluate blood and urine from dogs with MCTs to detect c-kit and its internal tandem duplication mutation in exon 11 compared to control dogs.
        701
            

























































   701   702   703   704   705