Page 710 - WSAVA2018
P. 710

 25-28 September, 2018 | Singapore
WSVA8-0188
OPHTHALMOLOGY
B-MODE ULTRASONOGRAPHIC ASPECTS AND BIOMETRY OF LEOPARD CAT (PRIONAILURUS BENGALENSIS)
T. Tangpakornsak1, N. Vorawattanatham2, A. Pinyopummin3, A. Thayananuphat4
1Kasetsart veterinary imaging and radiotherapy center, Kasetsart Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand
2Conservation and Research Section, Animal Management Division, Chiang Mai Night Safari, Chiang Mai, Thailand
3Department of Large Animal and Wildlife Clinical Science, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom, Thailand
4Department of Small Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Nakhon, Thailand
OBJECTIVES
To describe the normal ultrasonographic appearance and biometry of the leopard cat’s eye (Prionailurus bengalensis) using B-mode ultrasonography.
METHODS
8 leopard cats (Prionailurus bengalensis) from Chiangmai night safari with no history of previous ophthalmic disease were examined using the portable ultrasound machine with high frequency curvilinear transducer
METHODS
The demineralized freeze-dried bone allograft (DFDBA) was produced from feline (cadaver) bones. The long bones of extremities were used as raw materials. Both cancellous and cortical bones were collected and processed into 3 different sizes of 20, 40, and 60 mesh for each type of the bone. Sterilization of DFDBA was conducted using gamma radiation with doses of 15kGy and 25kGy to each type and size material produced resulting in 12 DFDBA variants. The bone graft produced then evaluated with the MTT assay using CPAE cells in D-MEM.
RESULTS
The results show that the highest cell viability (80,2%) lead by DFDBA derived from cortical bones with 20 mesh in size and 15kGy radiation dose. Followed by 20 mesh DFDBA derived from cortical bone sterilized by 25kGy with 72,8% cell viability.
CONCLUSIONS
From these results, we concluded that the CPAE cells growth was affected by the type of the bone, particle size, and radiation dose. The highest cell viability (lowest cell growth inhibition) was found in 20 mesh DFDBA derived from the cortical bone sterilized by 15kGy gamma radiation.
WSVA8-0077
ORTHOPEDICS
IN VITRO STUDY OF DEMINERALIZED FREEZE- DRIED BONE ALLOGRAFT STERILIZED BY GAMMA RADIATION AS BONE FILLER FOR COMMINUTED FRACTURE IN FELINE PATIENT
F. Amelia1, B. Abbas2, D. Darwis2, S. Estuningsih3, D. Noviana3
1Bogor Agricultural University, Graduate School, Bogor, Indonesia
2National Nuclear Energy Agency, Center for Isotope and Radiation Application, Jakarta, Indonesia
3Bogor Agricultural University, Department Clinic Reproduction and Pathology- Faculty of Veterinary Medicine, Bogor, Indonesia
INTRODUCTION
The incidence of fracture in cat, especially stray cats,
is quite high in Indonesia. Among the type of fractures, the comminuted fracture is still considered challenging to handle. In this case, the demineralized freeze-dried bone allograft (DFDBA) can be an option to replace the missing part of the bone.
OBJECTIVES
This research is aimed to evaluate cell viability of DFDBA by in vitro study using MTT assay.
    (10 MHz) in order to describe normal sonographic appearance and biometry measurement. These captive wild cats were premedicated and inducted with xylazine and zoletil, respectively. The anesthesia was maintained with isofurane.
RESULTS
The biometric findings were as follows; mean and standard deviation (Mean+SD) of the corneal thickness was 0.07+0.0006 cm Depths of the anterior and posterior chamber were 0.34 + 0.009 cm. Lens thicknesses were 0.81 + 0.014 cm. Depths of the vitreous chamber were 0.60 + 0.009 cm. No significant difference was found between the ocular biometry of the left and right eyes.
CONCLUSION
This study provides ultrasonographic appearance and baseline information of ocular biometry in leopard cat for further clinical investigations of ocular abnormalities using B-mode ultrasonography.
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43RD WORLD SMALL ANIMAL VETERINARY ASSOCIATION CONGRESS AND 9TH FASAVA CONGRESS





























































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