Page 718 - WSAVA2018
P. 718

 25-28 September, 2018 | Singapore
WSVA8-0131
SOFT TISSUE SURGERY
LOW DENSITY TUBULAR BOVINE COLLAGEN GRAFT FOR URETHRAL REGENERATION: PRELIMINARY CANINE MODEL
R. Radzi1, G. Vythilingam2, R.C. Thambidorai2,
T. Kamarul3, S. Sothilingam2, H.M. Larsson4,
E.M. Engelhardt4, E. Vardar5, K. Pinnagoda5, P. Frey4, J.A. Hubbell6, H.C. Chen1, M.W.H. Hiew1, N.H. Khairuddin1
1Universiti Putra Malaysia, Faculty of Veterinary Medicine, Serdang, Malaysia
2University of Malaya, Department of Surgery, Kuala Lumpur, Malaysia
3University of Malaya, Tissue Engineering Group- National Orthopedic Center of Excellence for Research and Learning NOCERAL- Department of Orthopedic, Kuala Lumpur, Malaysia
4Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
5Centre Hospitalier Universitaire Vaudois CHUV, Department of Pediatrics, Lausanne, Switzerland
6University of Chicago, Institute for Molecular Engineering, Chicago, USA
INTRODUCTION
Patients with urethral stricture and hypospadias require urethral reconstructive surgery. The US Food and Drug Administration (FDA) requires the demonstration of safety and efficacy of medical device in at least 2 animal models before clinical translation.
OBJECTIVES
This study is aimed to evaluate the efficacy of a tubular bovine collagen graft in urethral regeneration in a canine model.
METHODS
A 4 cm long urethral graft was surgically implanted in an artificially created urethral defect proximal to the baculum in a pilot study involving 3 dogs. A transurethral urinary catheter was kept post-op.
RESULTS
Dog 1 had successful implant procedure but compromised by iatrogenic urinary catheter removal. The complications were urine leakage, scrotal excoriation and cystitis. Dog 1 able to micturate normally following urinary catheter removal on post op day 14. Post op day 75, Dog 1 was euthanized. Histological data showed narrowing of the urethra at the distal anastomotic site
but patent urethra proximally. Both Dog 2 and 3 had catheters removed on post op day 10. They able to micturate normally. Ultrasound on post op week 6 revealed no stenosis. Contrast study on Dog C post op
5 months revealed patent urethra at the grafted site and narrowing of the urethra at the distal anastomotic site. No clinical micturition difficulty observed. To date (February 2018), Dog 2 and 3 are still under observation for long term graft complication.
CONCLUSIONS
This pilot study reveal canine is a promising model for urethral clinical translation studies and potential implant application in cases of canine urethral stenosis.
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43RD WORLD SMALL ANIMAL VETERINARY ASSOCIATION CONGRESS AND 9TH FASAVA CONGRESS








































































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