Page 721 - WSAVA2018
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WSVA8-0031
NUTRITION
ALTERATIONS IN GUT MICROBIOME BY THE PROTEIN TO CARBOHYDRATE RATIOS IN OBESE VS. LEAN DOGS
J. Li1
1Nestle Purina Research, 2S, St. Louis, USA
INTRODUCTION
An estimated 54% of dogs in the US are overweight or obese. Dysbiosis in gut microbes has been associated with obesity in humans and animal models. High protein low carbohydrate (HPLC) diets have been recommended for body weight management for decades, but their effects on canine gut microbiota is not well understood.
OBJECTIVES
To evaluate the effect of protein and carbohydrate ratios on gut microbiome in obese vs. lean dogs.
METHODS
63 Labrador Retrievers and Beagles, half obese or overweight (OW) and half lean or normal (LN), were fed the common baseline diet for 4 weeks, followed by 4 weeks of experimental diet: HPLC (49.4% protein, 10.9% carbohydrate) or low protein high carbohydrate diet (LPHC) (25.5% protein, 38.8% carbohydrate). Fecal samples collected at the end of baseline and experimental diets were subject to 16S rRNA gene sequencing analysis.
RESULTS
Diets predominantly exerted their effects on the two phyla, Bacteroidetes and Firmicutes. The effects were greater in OW dogs than in LN dogs, but independent of breed. The HPLC-fed dogs had decreased Bacteroidetes to Firmicutes ratios, and enriched microbial gene networks associated with weight maintenance, when compared with those on LPHC. The abundances of C. hiranonis, C. perfringens, and R. gnavus were higher
in the HPLC group, while B. uniformis and C. butyricum were enriched in the LPHC group.
CONCLUSIONS
This study provided an initial framework that will allow modulation of gut microbiota by nutrition interventions and may provide an alternative therapeutic option for canine obesity.
Your Singapore, the Tropical Garden City
WSVA8-0169
NUTRITION
DOCOSAHEXAENOIC ACID IN MICROALGAE SCHIZOCHYTRIUM SP. IN A WET PET FOOD IS STABLE DURING RETORT AND 24 MONTHS OF STORAGE AT AMBIENT TEMPERATURE
S. Yu1, D. Kappelman2, J. Alix3
1DSM Nutritional Products, Pet Nutrition, Parsippany, USA
2Blue Buffalo Pet Products- Inc., Research and Development, Wilton, USA
3DSM Nutritional Products, Global Marketing, Parsippany, USA
INTRODUCTION
Significant amounts of evidences have shown the health benefits of docosahexaenoic acid (DHA) in humans
and animals. Dried microalgae Schizochytrium sp. is a sustainable and commercially available ingredient with a high concentration of bioavailable DHA.
OBJECTIVES
The objective of this study was to measure the stability of DHA in a wet pet food during retort and storage.
METHODS
The dried microalgae Schizochytrium sp. (DHAgoldTM S17-B, DSM Nutritional Products, Columbia, MD, USA) was added at 2.3% in a typical loaf type of wet pet
food. It was mixed with chicken, chicken liver, chicken broth, brown rice, flaxseed, sweet potatoes, powdered cellulose, choline chloride, salt, potassium chloride, taurine, cassia gum, carrageenan, guar gum, vitamins and minerals in a mixer for 4 minutes and heated to 43 °C before filling the mixture in standard steel cans (156 g). The cans were sealed, retorted at 122 °C for 60 minutes, and cooled to room temperature before they were stored at 32 °C for 12 months and ambient temperature (21 °C) for 24 months. Samples were taken at various time points during the storage for DHA measurements with a GC method.
RESULTS
No noticeable loss of DHA was observed during the pet food process and retort. DHA retention were 66% at 12 months and 87% at 24 months under the storage conditions at 32 °C and ambient temperature, respectively.
CONCLUSIONS
In conclusion, DHA in DHAgold S17-B is stable in wet pet food during retort and long-term storage.
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